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e64-d (10 μm)  (Millipore)


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    Structured Review

    Millipore e64-d (10 μm)
    HPV16 infection is reduced in the presence of cysteine protease inhibitors . Infection of 293 cells alone, with HPV 16 DsRed reporter-virions (mock control, Infected), or in the presence of: 20 mM of lysosome neutralizing agent NH 4 Cl; 10 μM of a non-permeable cysteine protease inhibitor <t>E64;</t> 10 μM of the permeable cysteine protease inhibitor <t>E64-d;</t> 6 μM of the permeable intracellular cathepsin B inhibitor CA-074ME; or 10 μM of the cathepsin L inhibitor. Infection was analyzed and compared 48 hours post binding by FACS of DSRED expression. Inhibitors were present for the duration of infection. Cells alone were analyzed for background fluorescence. Statistics were analyzed by 1 tailed t-test and found to be significant at P < 0.05.
    E64 D (10 μm), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e64-d+%2810+%CE%BCm%29/pmc02718874-139-32-6?v=Millipore
    Average 90 stars, based on 1 article reviews
    e64-d (10 μm) - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "The role of NH 4 Cl and cysteine proteases in Human Papillomavirus type 16 infection"

    Article Title: The role of NH 4 Cl and cysteine proteases in Human Papillomavirus type 16 infection

    Journal: Virology Journal

    doi: 10.1186/1743-422X-6-109

    HPV16 infection is reduced in the presence of cysteine protease inhibitors . Infection of 293 cells alone, with HPV 16 DsRed reporter-virions (mock control, Infected), or in the presence of: 20 mM of lysosome neutralizing agent NH 4 Cl; 10 μM of a non-permeable cysteine protease inhibitor E64; 10 μM of the permeable cysteine protease inhibitor E64-d; 6 μM of the permeable intracellular cathepsin B inhibitor CA-074ME; or 10 μM of the cathepsin L inhibitor. Infection was analyzed and compared 48 hours post binding by FACS of DSRED expression. Inhibitors were present for the duration of infection. Cells alone were analyzed for background fluorescence. Statistics were analyzed by 1 tailed t-test and found to be significant at P < 0.05.
    Figure Legend Snippet: HPV16 infection is reduced in the presence of cysteine protease inhibitors . Infection of 293 cells alone, with HPV 16 DsRed reporter-virions (mock control, Infected), or in the presence of: 20 mM of lysosome neutralizing agent NH 4 Cl; 10 μM of a non-permeable cysteine protease inhibitor E64; 10 μM of the permeable cysteine protease inhibitor E64-d; 6 μM of the permeable intracellular cathepsin B inhibitor CA-074ME; or 10 μM of the cathepsin L inhibitor. Infection was analyzed and compared 48 hours post binding by FACS of DSRED expression. Inhibitors were present for the duration of infection. Cells alone were analyzed for background fluorescence. Statistics were analyzed by 1 tailed t-test and found to be significant at P < 0.05.

    Techniques Used: Infection, Protease Inhibitor, Binding Assay, Expressing, Fluorescence



    Similar Products

    e64-d (10 μm)  (Millipore)
    90
    Millipore e64-d (10 μm)
    HPV16 infection is reduced in the presence of cysteine protease inhibitors . Infection of 293 cells alone, with HPV 16 DsRed reporter-virions (mock control, Infected), or in the presence of: 20 mM of lysosome neutralizing agent NH 4 Cl; 10 μM of a non-permeable cysteine protease inhibitor <t>E64;</t> 10 μM of the permeable cysteine protease inhibitor <t>E64-d;</t> 6 μM of the permeable intracellular cathepsin B inhibitor CA-074ME; or 10 μM of the cathepsin L inhibitor. Infection was analyzed and compared 48 hours post binding by FACS of DSRED expression. Inhibitors were present for the duration of infection. Cells alone were analyzed for background fluorescence. Statistics were analyzed by 1 tailed t-test and found to be significant at P < 0.05.
    E64 D (10 μm), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e64-d+%2810+%CE%BCm%29/pmc02718874-139-32-6?v=Millipore
    Average 90 stars, based on 1 article reviews
    e64-d (10 μm) - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    HPV16 infection is reduced in the presence of cysteine protease inhibitors . Infection of 293 cells alone, with HPV 16 DsRed reporter-virions (mock control, Infected), or in the presence of: 20 mM of lysosome neutralizing agent NH 4 Cl; 10 μM of a non-permeable cysteine protease inhibitor E64; 10 μM of the permeable cysteine protease inhibitor E64-d; 6 μM of the permeable intracellular cathepsin B inhibitor CA-074ME; or 10 μM of the cathepsin L inhibitor. Infection was analyzed and compared 48 hours post binding by FACS of DSRED expression. Inhibitors were present for the duration of infection. Cells alone were analyzed for background fluorescence. Statistics were analyzed by 1 tailed t-test and found to be significant at P < 0.05.

    Journal: Virology Journal

    Article Title: The role of NH 4 Cl and cysteine proteases in Human Papillomavirus type 16 infection

    doi: 10.1186/1743-422X-6-109

    Figure Lengend Snippet: HPV16 infection is reduced in the presence of cysteine protease inhibitors . Infection of 293 cells alone, with HPV 16 DsRed reporter-virions (mock control, Infected), or in the presence of: 20 mM of lysosome neutralizing agent NH 4 Cl; 10 μM of a non-permeable cysteine protease inhibitor E64; 10 μM of the permeable cysteine protease inhibitor E64-d; 6 μM of the permeable intracellular cathepsin B inhibitor CA-074ME; or 10 μM of the cathepsin L inhibitor. Infection was analyzed and compared 48 hours post binding by FACS of DSRED expression. Inhibitors were present for the duration of infection. Cells alone were analyzed for background fluorescence. Statistics were analyzed by 1 tailed t-test and found to be significant at P < 0.05.

    Article Snippet: The following inhibitors were obtained from Calbiochem (Gibbstown, NJ) and used at the following non-toxic concentration: CA-074Me (6 μM), Z-FF-FMK an irreversible cell permeable cathepsin L inhibitor (10 μM), E64 (10 μM), E64-d (10 μM).

    Techniques: Infection, Protease Inhibitor, Binding Assay, Expressing, Fluorescence